Immunomodulatory Effects of Bio-Clean II on Total and Differential Leucocyte Counts in Rats Exposed to Purified Bacterial Lipopolysaccharide
Received Date:May 20, 2022; Published Date: July 06, 2022
Bio-Clean II has been previously shown to possess anti-inflammatory properties by regulating the serum level of inflammatory cytokines, C-reactive protein, Corticosterone, antiphospholipid antibodies, as well as T-Helper 4 and Cytotoxic T-Lymphocytes in rats exposed to purified bacterial lipopolysaccharide (LPS). In this study, we investigated the effects of Bio-Clean II on total and differential leucocyte counts in rats exposed to bacterial LPS. Thirsty six (36) male Wistar rats (150±50g) were divided into six (6) groups (n=6). Group 1, 2, 3, 5 and 6 were induced with a single dose of 5mg/Kg of purified Lipopolysaccharide from (E. coli O127:B8) intraperitoneally, except the Zero control (group 4) that was given feed and water only. The positive control (group 2) received 50mg/Kg/bid Diclofenac + 500mg/kg/bid of Ciprofloxacin, Negative control (group 3) received Sterile Phosphate Buffer Saline, while group 5 and 6 received Bio-Clean II for duration of 7 and 14 days, respectively. The rats were sacrificed by cervical dislocation afterwards, whole blood was collected and assayed for total white blood cell count (WBC), absolute lymphocyte count (LYM#), absolute neutrophil count (NEUT#), as well as absolute counts of the summation of monocytes, eosinophils and basophils (MXD#) using Sysmsex Haematology Autoanalyzer. The results of the study shows that the WBC (5.68±0.91 X103/uL), LYM# (4.04±0.85X103/uL), and NEUT# (1.10±0.11X103/uL) of the 7days (not 14 days) Bio-Clean II treated rat was found to be significantly lower when compared to the inflammation control group: 12.52±1.04 X103/uL (p=0.018), 7.66±0.75X103/uL (p=0.004) and 4.40±0.81X103/uL (p= 0.027), respectively. The outcome of this study shows that Bio-Clean II reversed the leukocytosis, lymphocytosis and neutrophilia associated with LPS-induced inflammation. This underscores the anti-inflammatory potential of Bio-Clean II in the treatment of bacterial inflammatory diseases. While data generated from this study may be extrapolated to humans, further studies are required to understand the molecular mechanism behind these effects.
Keywords: Bio-Clean II; Herbal remedy; Leucocyte counts; Lipopolysaccharides; Rats