Open Access Research Article

Immunomodulatory Effects of Bio-Clean II on Total and Differential Leucocyte Counts in Rats Exposed to Purified Bacterial Lipopolysaccharide

Seyi Samson Enitan1*, Isaiah Nnanna Ibeh2, Ayodeji Olusola Olayanju3, Emmanuel Olusegun Ileoma1, Abiodun Durosinmi4, Aderanti Adedotun Afeez5, Nkem Judith Adeyombo6, Ayomide Ruth Olabanji1

1Department of Medical Laboratory Science, Babcock University, Ilishan-Remo, Nigeria

2Department of Medical Laboratory Science, University of Benin, Nigeria

3Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Nigeria

4Department of Haematology, State Hospital, Ijebu-Ode, Nigeria

5Department of Medical Laboratory Science, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

6Department of Haematology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria

Corresponding Author

Received Date:May 20, 2022;  Published Date: July 06, 2022


Bio-Clean II has been previously shown to possess anti-inflammatory properties by regulating the serum level of inflammatory cytokines, C-reactive protein, Corticosterone, antiphospholipid antibodies, as well as T-Helper 4 and Cytotoxic T-Lymphocytes in rats exposed to purified bacterial lipopolysaccharide (LPS). In this study, we investigated the effects of Bio-Clean II on total and differential leucocyte counts in rats exposed to bacterial LPS. Thirsty six (36) male Wistar rats (150±50g) were divided into six (6) groups (n=6). Group 1, 2, 3, 5 and 6 were induced with a single dose of 5mg/Kg of purified Lipopolysaccharide from (E. coli O127:B8) intraperitoneally, except the Zero control (group 4) that was given feed and water only. The positive control (group 2) received 50mg/Kg/bid Diclofenac + 500mg/kg/bid of Ciprofloxacin, Negative control (group 3) received Sterile Phosphate Buffer Saline, while group 5 and 6 received Bio-Clean II for duration of 7 and 14 days, respectively. The rats were sacrificed by cervical dislocation afterwards, whole blood was collected and assayed for total white blood cell count (WBC), absolute lymphocyte count (LYM#), absolute neutrophil count (NEUT#), as well as absolute counts of the summation of monocytes, eosinophils and basophils (MXD#) using Sysmsex Haematology Autoanalyzer. The results of the study shows that the WBC (5.68±0.91 X103/uL), LYM# (4.04±0.85X103/uL), and NEUT# (1.10±0.11X103/uL) of the 7days (not 14 days) Bio-Clean II treated rat was found to be significantly lower when compared to the inflammation control group: 12.52±1.04 X103/uL (p=0.018), 7.66±0.75X103/uL (p=0.004) and 4.40±0.81X103/uL (p= 0.027), respectively. The outcome of this study shows that Bio-Clean II reversed the leukocytosis, lymphocytosis and neutrophilia associated with LPS-induced inflammation. This underscores the anti-inflammatory potential of Bio-Clean II in the treatment of bacterial inflammatory diseases. While data generated from this study may be extrapolated to humans, further studies are required to understand the molecular mechanism behind these effects.

Keywords: Bio-Clean II; Herbal remedy; Leucocyte counts; Lipopolysaccharides; Rats

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