Research Article
Localization of Herpetic Viruses Patterns in Sinonasal and Nasopharyngeal Malignant and Benign Tumors
Marwa Mohammed Ali Jassim1, Majid Mohammed Mahmood1* and Saad Hasan Mohammed Ali2
1Department of Biology, Mustansiriyah University, Iraq
2Diseases Research Unit, University of Baghdad, Iraq
Majid Mohammed Mahmood, Department of Biology, College of Science, Mustansiriyah university, Baghdad, Iraq.
Received Date: August 31, 2019; Published Date: September 16, 2019
Abstract
Background: Sinonasal and Nasopharyngeal carcinomas are related to many environmental and genetic predisposing factors. Epstein Barr viruses are well known to induce tumorigenesis in proliferative epithelial tissues. HCMV plays an “oncomodulatory” role in the neoplastic process and can induce cellular responses that would provide a growth advantage for neoplastic cells.
Objectives: This study designed to determine the herpetic viruses’ patterns (punctuated, diffused and mixed) in tissues from Nasopharyngeal and sinonasal lesions.
Methods: The study included a total number of (183) formalin fixed, paraffin-embedded tissue blocks including 35 inflammatory nasal polyps (INP), 35 sinonasal papillomas (SNP), 65 nasopharyngeal carcinomas (NPC), 18 sinonasal carcinomas (SNC) as well as 30 nasal healthy tissues as control group. After histopathological confirmatory re-examination, a Chromogenic in Situ Hybridization technique (CISH) for Epstein Barr virus encoded RNA (EBV-EBERs) and Human cytomegalovirus phosphoproteins 65 (HCMV-PP65) localization were performed.
Results: The mean (20.43) of EBV punctuators among tissues with malignant nasopharyngeal carcinoma was higher than the mean of punctuators (17.00) in sinonasal carcinoma group, whereas the mean of diffused form (71.89) in malignant sinonasal carcinoma tissues was higher than the mean (66.11) in nasopharyngeal carcinoma tissues. On the other hand, the mean of punctuated pattern in malignant sinonasal carcinoma tissues infected with HCMV was higher (21.56) than the means (16.94) of this pattern in nasopharyngeal carcinoma tissues, as well as the mean of diffused pattern in tissues with sinonasal carcinoma was higher (45.11) than its mean in HCMV infected nasopharyngeal carcinoma tissues (34.881). Conclusion: The present findings indicate the impact of both punctuated and diffused patterns in sinonasal and nasopharyngeal pathogenesis and carcinogenesis.
Keywords: NPC; SNC; EBV-EBERS; HCMV-PP65; CISH
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Majid Mohammed Mahmood, Marwa Mohammed Ali Jassim, Saad Hasan Mohammed Ali. Localization of Herpetic Viruses Patterns in Sinonasal and Nasopharyngeal Malignant and Benign Tumors. On J Otolaryngol & Rhinol. 1(5): 2019. OJOR.MS.ID.000524.