Open Access Research Article

Localization of Herpetic Viruses Patterns in Sinonasal and Nasopharyngeal Malignant and Benign Tumors

Marwa Mohammed Ali Jassim1, Majid Mohammed Mahmood1* and Saad Hasan Mohammed Ali2

1Department of Biology, Mustansiriyah University, Iraq

2Diseases Research Unit, University of Baghdad, Iraq

Corresponding Author

Received Date: August 31, 2019;  Published Date: September 16, 2019

Abstract

Background: Sinonasal and Nasopharyngeal carcinomas are related to many environmental and genetic predisposing factors. Epstein Barr viruses are well known to induce tumorigenesis in proliferative epithelial tissues. HCMV plays an “oncomodulatory” role in the neoplastic process and can induce cellular responses that would provide a growth advantage for neoplastic cells.

Objectives: This study designed to determine the herpetic viruses’ patterns (punctuated, diffused and mixed) in tissues from Nasopharyngeal and sinonasal lesions.

Methods: The study included a total number of (183) formalin fixed, paraffin-embedded tissue blocks including 35 inflammatory nasal polyps (INP), 35 sinonasal papillomas (SNP), 65 nasopharyngeal carcinomas (NPC), 18 sinonasal carcinomas (SNC) as well as 30 nasal healthy tissues as control group. After histopathological confirmatory re-examination, a Chromogenic in Situ Hybridization technique (CISH) for Epstein Barr virus encoded RNA (EBV-EBERs) and Human cytomegalovirus phosphoproteins 65 (HCMV-PP65) localization were performed.

Results: The mean (20.43) of EBV punctuators among tissues with malignant nasopharyngeal carcinoma was higher than the mean of punctuators (17.00) in sinonasal carcinoma group, whereas the mean of diffused form (71.89) in malignant sinonasal carcinoma tissues was higher than the mean (66.11) in nasopharyngeal carcinoma tissues. On the other hand, the mean of punctuated pattern in malignant sinonasal carcinoma tissues infected with HCMV was higher (21.56) than the means (16.94) of this pattern in nasopharyngeal carcinoma tissues, as well as the mean of diffused pattern in tissues with sinonasal carcinoma was higher (45.11) than its mean in HCMV infected nasopharyngeal carcinoma tissues (34.881). Conclusion: The present findings indicate the impact of both punctuated and diffused patterns in sinonasal and nasopharyngeal pathogenesis and carcinogenesis.

Keywords: NPC; SNC; EBV-EBERS; HCMV-PP65; CISH

Citation
Signup for Newsletter
Scroll to Top