Open Access Research Article

Development and Validation of Novel Spectro- Chemometric, Chemometric and TLC-Densitometric Methods for Simultaneous Determination of Timolol and Travoprost in their Bulk Powders and Pharmaceutical Formulation

Hesham Salem*1,3, Afaf Aboulkheir2 and Basma E Abdel Aziz3

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Deraya University, Egypt

2Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Egypt

3Faculty of Pharmacy, Deraya University, Egypt

Corresponding Author

Received Date: August 05, 2019;  Published Date: August 27, 2019

Abstract

Simple, sensitive and reliable spectro-chemometric, Chemometric and thin layer chromatography (TLC)-densitometric methods were developed for simultaneous determination of binary mixture of timolol (TIM) and travoprost (TRA) without prior separation in their bulk drugs and dosage forms. The speectro-chemometric methods used are derivative spectrophotometry and isosbestic point methods. Multivariate methods involves the application of three chemometric techniques; classical least square (CLS) principal component regression (PCR) and partial least-squares regression (PLS) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of TIM and TRA in presence of each other in bulk powder or in dosage form. Separation was carried out on merck HPTLC aluminium sheets of silica gel 60 F254 using ethyl acetate-methanol-ammonia (5:3:0.5) as mobile phase. All variables influencing the spectrophotometric and HPTLC methods were carefully investigated and optimized. The developed methods were evaluated according international conference of harmonization (ICH) rules. The current methods were successfully implemented for direct the determination of these drugs in their trade pharmaceutical formulations. The obtained results were contrasted with those calculated after application of reference method. The data obtained from both methods were statistically analyzed by Fand t-tests. The results of such study showed excellent agreement in respect to the accuracy and precision of the suggested and reference methods.

Keywords:Derivative spectrophotometry; Isosbestic point spectrophotometry; Multivariate chemometry

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