Open Access Research Article

DNA Interaction Study of Some Symmetrical 1,2-Phenylenediamine Schiff’s Base Derivatives as New Potential DNA Intercalators Using Ethidium Bromide Competition Fluorescent Assay

Sofian S Mohamed1, Inass A Sadawe1, Nisreen H Meiqal1, Abdulathim A Alshoushan2, Aisha S Aboud1, Safa A Aboulqasim1, Aisha A Issa1, Salah M Bensaber1, Anton Hermann3 and Abdul M Gbaj1*

1Department of Medicinal Chemistry, Faculty of Pharmacy, University of Tripoli, Libya

2Department Food and Drug Control Centre (LFDA), Tripoli, Libya

3Department of Biosciences, University of Salzburg, Salzburg, Austria

Corresponding Author

Received Date:July 12, 2019;  Published Date:July 18, 2019


A series of novel potential DNA intercalators of symmetrical 1,2-phenylenediamine Schiff’s base derivatives were synthesized. The binding properties of these compounds to genomic DNA (G-DNA) have been investigated by fluorescence spectroscopy. Ethidium binding to DNA significantly enhanced its fluorescence making it a convenient probe to evaluate DNA binding of many drugs. The addition of a DNA binding agent induces a progressive decrease in fluorescence of ethidium due to its displacement from the duplex. This also allows distinguishing non-intercalative binding agents from intercalating agents: agents having a huge site size groove binder need correspondingly smaller concentrations to saturate the sites. The results indicate that all the targeted compounds can interact with G-DNA, and among them, SW7 (1,1’-((1E,1’E)-(1,2-phenylenebis(azanylylidene)) bis(methanylylidene))bis(hthalen-2-ol)), showed the highest key selection vector (KSV) value, suggest that compound SW7, binds most strongly to G-DNA

Keywords: Fluorescence; DNA intercalation; Key selection vector

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