DNA Interaction Study of Some Symmetrical
1,2-Phenylenediamine Schiff’s Base Derivatives as
New Potential DNA Intercalators Using Ethidium
Bromide Competition Fluorescent Assay

Sofian S Mohamed; Inass A Sadawe; Nisreen H Meiqal; Abdulathim A Alshoushan; Aisha S Aboud; Safa A Aboulqasim; Aisha A Issa; Salah M Bensaber; Anton Hermann; Abdul M Gbaj

Research Article

DNA Interaction Study of Some Symmetrical 1,2-Phenylenediamine Schiff’s Base Derivatives as New Potential DNA Intercalators Using Ethidium Bromide Competition Fluorescent Assay

Sofian S Mohamed¹, Inass A Sadawe¹, Nisreen H Meiqal¹, Abdulathim A Alshoushan², Aisha S Aboud¹, Safa A Aboulqasim¹, Aisha A Issa¹, Salah M Bensaber¹, Anton Hermann³ and Abdul M Gbaj¹*

¹Department of Medicinal Chemistry, Faculty of Pharmacy, University of Tripoli, Libya

²Department Food and Drug Control Centre (LFDA), Tripoli, Libya

³Department of Biosciences, University of Salzburg, Salzburg, Austria

Corresponding Author

Received Date:July 12, 2019; Published Date:July 18, 2019

Abstract

A series of novel potential DNA intercalators of symmetrical 1,2-phenylenediamine Schiff’s base derivatives were synthesized. The binding properties of these compounds to genomic DNA (G-DNA) have been investigated by fluorescence spectroscopy. Ethidium binding to DNA significantly enhanced its fluorescence making it a convenient probe to evaluate DNA binding of many drugs. The addition of a DNA binding agent induces a progressive decrease in fluorescence of ethidium due to its displacement from the duplex. This also allows distinguishing non-intercalative binding agents from intercalating agents: agents having a huge site size groove binder need correspondingly smaller concentrations to saturate the sites. The results indicate that all the targeted compounds can interact with G-DNA, and among them, SW7 (1,1’-((1E,1’E)-(1,2-phenylenebis(azanylylidene)) bis(methanylylidene))bis(hthalen-2-ol)), showed the highest key selection vector (KSV) value, suggest that compound SW7, binds most strongly to G-DNA

Keywords: Fluorescence; DNA intercalation; Key selection vector

Article Details

Citation

Abdul M Gbaj, JSofian S Mohamed, Inass A Sadawe, Nisreen H Meiqal, Abdulathim A Alshoushan, Aisha S Aboud et al. DNA Interaction Study of Some Symmetrical 1,2-Phenylenediamine Schiff’s Base Derivatives as New Potential DNA Intercalators Using Ethidium Bromide Competition Fluorescent Assay. Arch Biomed Eng & Biotechnol. 2(2): 2019. ABEB.MS.ID.000534.