Open Access Research Article

Molecular Identification of Aerococcus viridans Associated with Bovine Mastitis and Determination of Antibiotic Susceptibilities

Kirkan Sukru*, Parin Ugur, Yuksel Hafize Tugba and Oklay Mehmet Ali

Department of Microbiology, Aydin Adnan Menderes University, Turkey

Corresponding Author

Received Date: September 23, 2018;  Published Date: October 09, 2018

Abstract

Aerococcus species are saprophytic in the environment as well as many human and animal infections. It has also been reported to be detected in clinical and subclinical mastitis cases in cattle. The similarity with Streptococcus and Staphylococcus species is known and may lead to misdiagnosis of Aerococcus species. In this research, 100 milk samples were collected from the farms in Aydin where clinical and subclinical mastitis was detected. The samples were brought to Aydin Adnan Menderes University Veterinary Faculty Microbiology Department by cold chain. A total of 15 (15%) Aerococcus viridans isolates were identified from clinical and subclinical mastitis cows with MALDI-TOF MS, which is an automated identification system, and genotypic identification were performed by targeting the 16S rRNA gene. In the antimicrobial susceptibility tests based on Minimal Inhibitor Concentration (MIC), the isolates were 100% sensitive to Ciprofloxacin, Tigecycline and Trimethoprim-sulfomethoxazole, 87% sensitive to Vancomycin, 80% sensitive to Erythromycin, 67% sensitive to Tetracycline, 53.3% sensitive to Penicillin and Linezolide; 93% resistant to Nitrofurantoin and 53.3% resistant to Teicoplanin. It is predicted that the obtained regional data will shed light on the role of A. viridans in pathogenesis of mastitis in cattle for future studies.

Keywords: Cattle; Aerococcus viridans; 16S rRNA; Mastitis; Identification

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