A Review on the Effect of Rooting Media on Rooting and Growth of Cutting Propagated Grape (Vitis vinifera L)

Grape (Vitis vinifera L.) is one of the important commercial subtropical vine crops grown all over the world except at a few places with high altitude and extreme temperature [1]. It is native to the Mediterranean region, central Europe, and southwestern Asia, from Morocco and Portugal north to southern Germany and east to northern Iran. It belongs to the family Vitaceae, is one of the oldest, most extensively cultivated and economically significant fruit crops in the world [2,3].

The three major uses of grapes are; wine making, fresh fruit (table grapes) and dried fruit (raisins) production. It can be used for making wine, jam, juice, jelly, grape seed extract, ethanol, raisins, vinegar, grape seed oil, tartaric acid, fertilizer, grape derived antioxidant compounds (polyphenols, resveratrol) and etc. Grape also associated with prevention of cancer, heart disease, high blood pressure, allergies, diabetes, constipation etc. [4].

It is a vine crop and trained on wires on both sides of plant. It is a short duration crop and consumed as fresh and in dried form [5]. Global grape production currently amounts to more than 75 million metric tons per year. Today there are over 18 million acres of cultivated vineyards worldwide. The top 5 grape producing counties are China, Italy, United States, France and Spain respectively, while Ethiopia is 77th in the world.

In Ethiopia, the total grape production, harvested area and yield of grape in the year 2014 was reached 5118 tones, 2544 ha and 20123 hectogram ha-1, respectively [6].

Grape propagation for commercial vineyards includes the use of cuttings, rooting, budding, layers and grafts [7]. Cutting is one of the extensively practiced means of vegetative propagation of plants in horticulture industry [8] and is the most important practices in viticulture [9]. It has many advantages such as being economical [1], require a limited space, simple [8,10], rapid for dissemination of selected clones or new varieties resulting from breeding programs [8,11]. It maintains true to type varietal characteristics [7,12,13]. Cutting is highly practical and economically important. It is used extensively to propagate ornamental plants, including deciduous types, broad-leaved evergreens and coniferous forms. Fruits such as grapes and figs have been propagated in this manner since ancient times [14].

Grapevines are very easy to grow from cuttings [8,13]. It is well known that in comparison to soft wood cuttings, grapes are generally propagated through hardwood cuttings [8]. The main reasons are due to its highest success rate [9,15] without the use of special rooting technique, least expensive and easiest method of vegetative propagation [12,13,16]. Cuttings can also be made from the stem, modified stem, roots or leaves [13].

Rooting media is one of the most important factors for rooted cutting production. It is one of the factors affecting rooting and growth of grape cuttings [4]. Types of media have significantly influenced the rooting and vegetative growth of cuttings. Growing media should be considered an essential part of the propagation system because rooting competency depends on the type of medium used. Rooting medium directly effect on quality and percentage of rooting [5]. Both the biological and physico-chemical characteristics of a potting medium affect plant and root growth [4]. It is known that good growth media provides a reservoir for plant nutrients, hold plant available water, and provide a means for gas exchange and good anchorage for the plants [17].

• To review the effect of different growth media on rooting percentage, roots and shoot growth performance of grape cutting.

• To identify research gap on the effect of growth media on rooting and required growth performance of grape cutting.

The quality of potting mixes and field nursery soils is critical to cutting establishment [13]. It is known that good growth media provides a reservoir for plant nutrients, hold plant available water, and provide a means for gas exchange and good anchorage for the plants [17-19]. Lack of one or more of these beneficial characteristics leads to lower rooting percentage of cuttings or undesirable root shape and or form [18]. Thus, growers typically use peat, perlite, vermiculite, sand, fallow land and organic and inorganic composted materials to prepare nutritious potting mixtures [20].

Review on the effect of media on rooting and root growth parameters of grape cutting

Effect of media on rooting percentage of grape cutting: Factors affecting rooting of grape cuttings can be internal or external factors. Internal factors affecting rooting of cuttings include the amount of stored food in cuttings, the age and maturity of tissue, the formation of callus and adventitious roots and the presence of leaves and buds on cuttings. The external factors include rooting media, chemical and hormone treatments, light, temperature, mechanical treatment and mist spray [14].

Many papers present studies on the effect of various media for rooting cuttings. Vermiculite, perlite, and other products have been and are being tested with a view of improving plant propagation methods [14].

Many mixtures have been used as media for propagation. Cuttings of some plants which root poorly in sand, often root satisfactorily in mixtures of equal volumes of sand and peat. A mixture of equal parts of peat and sawdust was satisfactory for rooting of grape. A mixture which contained 1 part of peat, 1 part of sand, and 1 part of sawdust also proved satisfactory [21]. Any medium which holds moisture and supplies air is satisfactory. However, different media cause variations in root quality [22]. Of 43 kinds of plants propagated by stem cuttings, 30 produced finer and more flexible roots in peat moss than in sand due to the reduced aeration and increased moisture [14].

When cuttings are rooted in sand and peat moss or perlite and peat moss, the roots developed are well branched, slender and flexible, a type much more suited for digging and repotting [23]. Among some of the rooting media used in Ghana is a mixture of equal parts of coarse river sand and composted oil palm fiber. The fiber holds moisture while the sand keeps the mixture open and well aerated [24].

Tsipouridis et al. [25] studied on five rooting substrates (perlite (1-5 mm), peat, perlite + peat (50:50 %), sand and perlite (covered cuttings were additionally enclosed in a polyethylene bag)) and found rooting the 50:50 peats perlite mixtures gave a reasonable amount.

Dvin et al. [18] also reported that using of coco peat + perlite media resulted in higher percentage of cuttings that rooted.

Ibrahim [24], showed that sand/ fiber mixture gave a higher percentage of rooting success and produced stronger and more fibrous roots than sand, fiber or peat moss alone.

Muhammad et al. [5] observed that the statistical analysis depicts significance of potting media on the rooting percentage (P<0.05). CSb and CSBCP potting media had more than 70% rooting in comparison to CS and CSFYM potting media with less than 50%. The highest mean rooting percentage (84.44) was observed from grape cuttings grown in potting media having mixture of canal silt (25%), bagasse (50%) and coco peat (25%).

Research done by Krishna [1] as in (Table 1) better rooting obtained from sand + 10 or 20% coco peat for hardwood cuttings of both Dogridge and 1613C. While in case of soft wood cuttings, sand + 10% coco peat recorded significantly higher percentage of rooting in both varieties (Table 1).

Ferrer et al. [26] reported that, percentage rooting was highest in sand (84.9), followed by soil (37.7) and the soil + sand mix (27.8). But the root development in sand was poor and the plants were not commercially acceptable. While the cuttings rooted in soil + sand mix produced better root and shoot development.

Table 1:Effect of different proportions of sand and coco peat on percentage of rooting in hardwood cuttings of grape.

Kawecki & Kozlowski [27] studied the effect of nine different organic substrates viz., municipal waste compost, conifer bark, peat, sand, fresh or old conifer sawdust, and hardwood sawdust in different combinations on rooting of one-bud hardwood cuttings of grape cv. Skarb Panoii. They found best rooting after five weeks on a substrate containing 8-year-old sawdust + sand or sand mixed with lowland peat.

Cyrillo et al. [28] observed no difference between two rooting media viz., vermiculite and washed river sand when used for rooting of semi hardwood cuttings of the grape rootstocks, ‘IAC 313 Tropical’ and ‘IAC 766 Campinas’ in a mist chamber.

Song et al. [29] observed sand + vermiculite as best basic substrate for rooting of cuttings in four grape varieties derived from crosses involving Vitis amuriensis.

Haile [4] Cutting rooted in agricultural soil + sand (75:25 v/v) gave the highest percent of cuttings that rooted (85.96 %), however, statistical difference was not observed between agricultural soil (100 %) and coco peat (100%) media. Similar result was reported by Sabir et al. [19], Dvin et al. [18], Sengel et al. [30] and Galavi et al. [17] in that rooting percentage of cuttings was significantly affected by the type of rooting media used.

Effect of media on number of roots per cutting: Muhammad et al. [5] done research using four media mixtures a. CS - canal silt (100%), b. CSFYM - canal silt (75%) + FYM (25%), c. CSB - canal silt (25%) + bagasse (75%) and d. CSBCP - canal silt (25%) + bagasse (50%) + coco peat (25%)(w/w) and the statistical analysis reveals that number of roots per cutting was significantly affected by the potting media and the higher mean numbers of roots (50.00) were obtained in CSBCP potting medium.

Research done by [1] using 4 treatments T1 Sand T2 : Sand + 10% coco peat T3 : Sand + 20% coco peat T4 : Sand + 30% coco peat with two types of cutting (semi hard and hard wood cutting) and 2 varieties indicated the number of roots per cutting recorded were greater in sand + 20% coco peat.

Singh & Nair [11] reported the effect of rooting media on root formation in cuttings of ornamental plants, planted in red soil, sand and compost in the ratio of 1:2:1, 1:1:2 and 2:1:1. Maximum root number was observed in media red soil + sand + compost (1:1:1).

As the observation of Gebreslassie [31] the largest number of roots per cutting (18) was recorded under rooting media composition of 6TS:3FYM :2FS.

In Rashad et al. [32] experiment different growing media such as Peat moss: Sand: Sawdust (1:1:1), Silt, Top soil and Bagasse: Silt (1:1) and different cutting height (Bottom, Middle and Top) were evaluated by using single concentration of IBA (400 mg kg-1) on guava. Results indicated that silt media had recorded significantly higher number of roots (28.78±3.99).

Effect of media on fresh weight of roots cutting: The study of Muhammad et al. [5] indicated that fresh weight of roots per cutting was significantly affected by the potting media. Results depicts that the highest fresh weight of roots per cuttings (2.16) was observed from the cuttings planted in CSB potting medium having mixture of canal silt (25%) and bagasse (75%). They further concluded that combination of canal silt (25%) and bagasse (75%) (CSB potting medium) as well as CSBCP [canal silt (25%) + bagasse (50%) and coco peat (25%) potting medium] had produced best results for sprouting and growth of grape seedlings.

In Haile’s (2017) study, root fresh weight was significantly (P≤0.01) influenced by rooting media. Stem cuttings rooted in agricultural soil + sand (75:25 v/v) gave significantly (Table 2) heavier (15.30 g) fresh root mass, while the minimum root mass (12.45g) was found in those cuttings rooted in agricultural soil (100%), which however was not statistically different from those cuttings rooted in filter cake + sand (50:50v/v) and coco peat (100%). Galavi et al., [17] also noted that root fresh weight of grape rooted cuttings was higher in agricultural soil + sand planting bed than pure sandy bed and agricultural soil beds.

Shah et al. [33] also reported significant effects of rooting media on root fresh weight of rooted cuttings.

In an experiment, Hong et al. [34] used different media mixtures viz., 100 per cent vermiculite, 100 per cent granulated cotton, 70 per cent vermiculite + 30 per cent perlite, 70 per cent vermiculite + 30 per cent peat moss and 50 per cent vermiculite + 50 per cent perlite for the acclimatization of in vitro cultured grape cv. Rizamat plantlets and found that 100 per cent granulated cotton followed by 70 per cent vermiculite + 30 per cent peat moss were best. The root weight was best in 70 per cent vermiculite + 30 per cent peat moss.

Gebresilassie [31] report shows that the highest fresh weight (30g) was recorded from hard wood cutting type grown in media composed of l/3rd SS top + 2TS:1FYM :1FS.

Effect of media on root length per cutting of grape: Singh and Nair [11] reported that the longest root length was observed in media red soil + sand + compost (1:1:1).

Haile [4] recommended agricultural soil + sand (75:25 v/v) out of the 4 rooting media i.e. agricultural soil (100%), agricultural soil+ sand (75:25v/v), filter cake + sand (50:50 v/v) used for longest (29.72 cm) root length (Table 2).

Table 2: Root growth parameters of grape cuttings as affected by rooting media and variety.

Dvin et al. [18], also found that significantly longer roots on apple hardwood cuttings (MM111) rooted in coco peat + perlite medium. However, Sengel et al. [50] reported non-significant effect of rooting media on root length.

Hong et al. [34] used different media mixtures viz., 100 per cent vermiculite, 100 per cent granulated cotton, 70 per cent vermiculite + 30 per cent perlite, 70 per cent vermiculite + 30 per cent peat moss and 50 per cent vermiculite + 50 per cent and He found root length was highest in 100 per cent granulated cotton.

Research done by [1] using 4 treatments T1 Sand T2 : Sand + 10% coco peat T3 : Sand + 20% coco peat T4 : Sand + 30% coco peat with two types of cutting (semi hard and hard wood cutting) and 2 varieties indicated them maximum length of longest root was recorded in sand + 30% coco peat in both hardwood and semi hard wood cuttings.

In Rashad’s et al. [32] experiment (Peat moss: Sand: Sawdust (1:1:1), Silt, Top soil and Bagasse: Silt (1:1) and different cutting height (Bottom, Middle and Top)) with single concentration of IBA (400 mg kg-1) on guava, Silt media had recorded significantly higher root length (24.95±5.00 cm).

Effect of media on growth of grape

The growth and survival of the grape seedlings in a nursery is greatly affected by the potting medium. As it is a key source of nutrition and provides root system to the plants. Besides, water holding capacity, better aeration, root penetration, presence of organic matter in the growing medium and so many other related factors are greatly influenced by the growing medium. A good potting medium must be easy to supply, process and a cheap source [35].

Different planting media have significantly influenced the vegetative growth of cuttings; due to the level of organic matter content, water holding difference [19]. The level of nitrogen and potassium in rooting media also plays a vital role in vegetative growth of the plant and improving the water utilization capacity of plant, respectively [30].

Effect of media on number of leaves per cutting of grape: Muhammad et al. [5] observed maximum number of leaves per stem cutting (17.00) from the stem cuttings planted in the potting medium had mixture of canal silt (25%) and bagasse (75%). Statistically similar results were also observed from the cuttings grown in the medium contained canal silt, bagasse and coco peat at 25, 50 and 25% respectively.

Haile [4] indicated that stem cutting rooted in filter cake + sand (50:50v/v) gave on average of 21.42 leaves plant-1, which however was not statistically different from those cuttings rooted in agricultural soil + sand (75:25v/v) (Table 3). Shah et al. [33] and Popescu & Popescu [20] also described that the significant effect of rooting media on number of leaves plant^-1.

Table 3:Effects of potting media and grape variety on growth indices of grape cuttings.

The experiment conducted by Gebreslassie [31] indicated that the highest average number of leaves with values (20) per stem cuttings were counted for hard wood cutting types for media proportion 2TS:1FYM:1FS.

Rashad et al. [32] also reported that Silt media had recorded significantly higher number of leaves (4.88±0.53). The research done by Ankita [36] identified that the maximum leaves per cutting is obtained in T1(soil) media. The research done by Dhatrika [37] showed that, the highest number of leaves per cutting was recorded in coco peat.

Effect of media on Shoot length of grape: Haile [4] also observed that the stem cuttings rooted in filter cake + sand (50:50v/v) showed significantly larger average shoot length (26.81 cm) yet statistically not different from those cuttings rooted in agricultural soil + sand (75:25v/v), whereas those cuttings rooted in coco peat (100%) gave the shortest (12.80 cm) average shoot length.

The report of Gebreslassie [31] also shows that longest shoot length (361cm and 350 cm) was recorded for soft wood (SW) cutting and hard wood (HW) cutting grown in rooting media 6TS: 3FYM: 2FS proportion.

Rashad et al. [32] also reported that silt media had recorded significantly higher shoot length (26.86±4.63 cm).

The research done by Ankita [36] using 14 treatments: {T1:soil,T2: coco peat, T3:vermicompost,T4:sand,T5:FYM,T6:- soil+sand(1:1),T7:soil+vermicompost(1:1), T8:soil+cocopeat (1:1), T9:soil + FYM(1:1), T10:soil + sand + FYM(1:1:1), T11: soil + sand + vermin compost (1:1:1), T12: soil + sand + cocopeat(1:1:1), T13:sand + FYM + cocopeat (1:1:1), T14: sand +vermin compost + coco peat (1:1:1)} that the maximum leaf length in T4(sand) media.

Effect of media on Leaf area (LA) of grape cutting: As the study of Haile [4], Leaf area was significantly (P≤0.01) influenced by rooting media. Cuttings rooted in filter cake + sand (50:50v/v) gave on average of 49.84 cm2 LA, which however was not statistically different from those cuttings rooted in agricultural soil + sand (75:25v/v).

Similar results were reported by Shah et al. [33] and Popescu & Popescu [20] in that leaf area of rooted cuttings were influenced by the type of rooting media used.

As the report of Gebreslassie [31] the larger leaf area (5513cm) was recorded for hard wood cutting grown in 2TS:1FYM:2FS media proportion. Ankita (2012) founded maximum leaf length in sand media and maximum leaf breadth in soil + sand + cocopeat, 1:1:1.

Effect of media on Shoot fresh weight of grape cutting: Haile [4] has also studied the shoot fresh weight of the cutting. In his study he found that the highest shoot fresh weight (25.28 g) was obtained from those cuttings rooted in agricultural soil (100%), which however was not statistically different from those cuttings rooted in filter cake + sand (50:50 v/v). Sabir et al. [19] and Senegel et al. [30] also noted that shoot fresh weight was significantly determined by the type of rooting media.

Gebreslassie [31] also observed the highest average fresh shoot weight (163g and 160 g) was recorded for semi hard wood cutting grown in rooting media 2TS:1FYM:1FS and soft wood cutting grown in rooting media 2TS:1FYM:2FS, respectively.

Rashad et al. [32] reported that the silt media had recorded significantly higher fresh weight of guava cutting (877.57±27.26 mg).

Effect of media on shoot thickness of grape cutting: Haile [4] also observed that cuttings rooted in filter cake + sand (50:50v/v) gave thicker (5.23 mm) shoots yet statistically not different from those cuttings rooted in agricultural soil + sand (75:25 v/v) (Table 3). The findings of Sabir et al. [19] also indicated that highest shoot thickness in one bud grape cuttings rooted in a sand media.

Somkuwar et al. [38] concluded that an increase in cane diameter on basal portion of cuttings (1-4 buds) is because of the accumulation of large amount of reserve food materials.

As the report of Gebreslassie [31] the highest average shoot girth (2.23 mm) was recorded for semi hard wood cutting type and 6TS: 3FYM:2FS media proportion. In Rashad et al. [32] experiment silt media had recorded significantly higher stem diameter (5.30±0.65 mm).

Effect of media on survival percentage of grape cutting: Sharma [39] conducted an experiment on fifteen types of rooting media and maximum success rate of 47% and survival percentage of 41% was found in media FYM + soil.

Lakra [40] experimented in Horticulture Nursery, BAU, Kanke on semi hardwood cuttings of passion fruit on 12 types of rooting media [41,42]. The maximum shoot length (20.07 cm) was observed in FYM + soil media. Firoz et al. [43] identified the highest success rate (79.1 and 80.2%), with the media containing 50% sand and 50% cow dung followed by media containing soil: sand: cow dung (1:1:1).

Singh & Nair [11] reported the effect of rooting media on cuttings of ornamental plants in red soil, sand and compost (1:2:1), (1:2:2) and (2:1:1). Maximum percent of success was obtained in media red soil: sand: compost (1:2:1) that is 100%.

In Rashad et al. [32] experiment silt media had recorded significantly higher survival percentage (83.33±16.33%). Dhatrika [37] also observed that survival percentage of rooted cuttings was higher in coco peat media.

Grape vines are very easy to grow from cuttings and generally propagated through hardwood cuttings [44-47]. Adventitious rooting and shoot growth are affected by environmental conditions, exogenous and endogenous biochemical compounds, ontogenetic age of plant material, genotype, characteristics of the cuttings i.e. growth conditions of the stock plant, rooting media and treatment of cuttings.

Types of media have significantly influenced the rooting and vegetative growth of cuttings. Growing media should be considered an essential part of the propagation system because rooting competency depends on the type of medium used. Rooting medium directly effect on quality and percentage of rooting [48-52]. Both the biological and Physico-chemical characteristics of a potting medium affect plant and root growth.

Choosing the most suitable growing media for the achievement of a successful plant production is very important. The growth and survival of the grape seedlings in a nursery is greatly affected by the potting medium. As it is a key source of nutrition and provides root system to the cuttings of the plants. Besides, water holding capacity, better aeration, root penetration, presence of organic matter in the growing medium and so many other related factors are greatly influenced by the growing medium [53-57].

From the review we can conclude that growing media significantly influenced the rooting, root and shoot growth performance of grape stem cutting.

The suitability of the rooting medium depends on the species, type of cuttings, growing conditions, season of the year and the cost effectiveness of the medium components. A media which is light, rich, porous, well drained and free from pathogens is considered ideal for growing of grape. A good potting medium must be easy to supply, process and a cheap source.

In general, we can conclude that the mixture of organic and inorganic media is very important for the rooting, root growth and shoot growth of grape propagated by cutting.

• Research should focus on the preparation of mixture of media from locally available materials.

• Research output is also premium important on the use of existing commercial mixtures.

• More commercial mixtures should be researched and be available for the end users.

• Integration study of many organic and inorganic growth media is paramount important.

None.

No conflict of interest.

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