The Presence of GFP in Embryos and its Effect on Cells
Received Date:July 25, 2022; Published Date: August 30, 2022
GFP is a 238 amino acid ~27 kDa protein derived from the crystal jellyfish Aequorea Victoria. was used to produce transgenic chickens, transgene expression was primarily in the pancreas and to a lesser extent in the liver, skin, muscles, and intestinal lining. The study describes the expression of GFP in various tissues from fetal, neonatal and adult animals. GFP expression in brain, lung, liver, and islet tissues was limited early in development, but was consistently strong in exocrine pancreas, kidney, cardiac, and skeletal muscle. The CAG promoter, which was hypothesized to induce ubiquitous protein expression, may be responsible for the differences in expression.
Keywords: GFP; Embryo; Cell; Amino acid; Transgenic; Protein