Research of Oxidative Stress and Serum Thiols as a Criterion for the Antioxidant Barrier in Patients with Heart Failure (NYHA FC III-IV)
Received Date: April 30, 2020; Published Date: May 11, 2020
Aim: To evaluate the status of oxidative stress and antioxidant systems (thiol and biological plasma antioxidant potential) in patients with heart failure, combined with disorders of carbohydrate metabolism and renal failure, and to what extent their values depend on the duration and severity of the disease (by NYHA).
Materials and Methods: Forty subjects (age 66.4±6.8) admitted for exacerbated chronic heart failure (III and IV FC), and 12 of them were examined in the same age range without clinical and echocardiography for HF. The levels of total thiols (SHp), reactive oxygen metabolites (dROMs), plasma antioxidant potential (BAP), creatinine, calculated GFR, glucose and C-reactive protein are analysed. Measurement of SHp, dROMs and BAP assays was performed spectrophotometrically with kits of Diacron International-Italy with an Olympus AU400 biochemical analyser and Carpe diam analyser (Diacron).
Results: An increase in oxidative stress was observed in the patient group, which had a high negative correlation with the thiol groups and the plasma antioxidant potential (r=-0.7404; r=-0.7567). The decrease in glomerular filtration also corresponds to a high degree with the reduction of the organism’s antioxidant capacity and the increase of oxidative stress. We found a high degree of dependence on the severity of HF (according to the NYHA classification), with the studied levels of oxidative stress and the antioxidant barrier. A similar relationship is observed between disease duration and SHp, dROMs and BAP concentrations.
Conclusion: Oxidative stress plays an essential role in the pathogenesis and progression of CHF. The observed thiol levels have decreased statistically significant, such as the collapse of the antioxidant system is key in the disturbed homeostasis of organisms in ROS/RNS coping.
Keywords: Thiols; Oxidative stress; Antioxidant system; Heart failure; GFR, Inflammatory response.