The Effects of Tyrosine-Protein Kinase Kit on Bronchopulmonary Dysplasia

Bronchopulmonary dysplasia (BPD) is a multifactorial chronic pulmonary disorder which complicates multiple pulmonary hypertensions in preterm infants. At present, there are no effective prevention or treatment options for BPD in clinical practice. Tyrosine-Protein Kinase Kit (KIT) serves an important role in regulating cell proliferation, hematopoiesis and stem cell maintenance. In the present study, the protective role of overexpression of KIT in BPD was investigated. The candidate differentially expressed genes (DEGs) between patients with BPD and healthy controls were screened using bioinformatics. A neonatal BPD mouse model was established under a hyperoxic environment and KIT overexpressing cells were intravenously injected into the mice, followed by evaluation of the effects on respiratory system resistance, pulmonary development and remodeling. Bioinformatics analysis showed that KIT was down regulated in patients with BPD, and a protein-protein interaction network was created using the Search Tool for Recurring Instances of Neighboring Genes database, which indicated that KIT was associated with known diseaserelated genes and regulated VEGF expression. In neonatal BPD mice, KIT exhibits significant protective affects and may thus serve as a candidate therapeutic target for treatment of patients with BPD treatment.

Keywords: Bronchopulmonary dysplasia; Tyrosine-Protein Kinase Kit; Vascular endothelial growth factor; Pulmonary fibrosis, type II collagen; CD31