Open Access Research Article

Influence of Different Counting Parameters for the Measurement of Thyroid and Thyroid Related Hormones Using Radio Immuno Assay (RIA) Technique and 125I Isotopes

Md Anwarul Kabir Bhuiya1*, Raihana Ferdaws1, Abu Mahmud1, Md Abdul Halim1, Md Shahnewaz Parvez1, Shefali Khatun2, Nasrin Laz2 and Md Shariful Islam Chowdhury2

1Department of Materials Science and Engineering, University of Rajshahi, Rajshahi, Bangladesh

2Institute of Nuclear Medicine and Allied Sciences (INMAS), BAEC, Rajshahi, Bangladesh

Corresponding Author

Received Date: March 10, 2019;  Published Date: May 07, 2019


Different methods for the determination of thyroid and related hormone have been reviewed. Performance characteristics of the radio isotopic method for the hormone assay were identified. Bound T3, Bound T4, TSH and Free T4 were assayed using different counting time and two different pulse height window levels. A very little difference was found during the measurement. The whole procedure was performed using Canberra AccuSpec NaI system, which has a high precision and stability. (c Squire equals to 3.56, detector resolution 9.10% and a 2k channel MCA). It is noticed that for low counting measurement counting time and window effect are present. It is assumed that for analog devices with single channel system the difference may be significant.

Keywords:Radio Immuno Assay (RIA), Counting time; Window levels; Radioactive Materials; Gamma Camera; Thyroid Hormones

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