Antibacterial Activity of Honey Against Staphylococcus Aureus and Pseudomonas Aeruginosa Isolated from Infected Wound

Wound infections have been recognized as the most critical problem especially in the presence of foreign materials that increase the risk of serious infection even with relatively small bacterial infection. This study was carried out to determine the antibacterial activity of honey against clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa obtained from infected wounds. Different concentrations of honey extract (25, 50, 75 and 100v/v) were tested using agar well diffusion method to determine their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa isolated from infected wounds of patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano. The antibacterial activity of the honey showed that the honey demonstrated antimicrobial activity against the test isolates with higher activity in Staphylococcus aureusM (with average zone of inhibition of 15.90mm) than Pseudomonas aeruginosa (14.63mm). The minimum inhibitory concentration (MIC) of the extract from this study ranges from 6.25-50mg/ml while the minimum bactericidal concentration (MBC) of the extracts ranges from 25-50mg/ ml. Statistical analysis of the results showed that there is no significant different in the activity of honey against the isolates at p< 0.05. Based on the results of this study, it is concluded that honey is active against wound bacterial isolates and can be used as a therapy for wound infection.

Keywords: Antibacterial activity, Honey, Pseudomonas aeruginosa, Staphylococcus aureus, Wound