Polyamide Dendrimers and Spermine Interact with

DNA

Ali Elejmi; Asma Abdalgader; Wael Elhrari; Inass A Sadawe; Inass A Sadawe; Jamal Ali Elbakay; Salah M Bensaber; Abdul M Gbaj; Abdulathim A Alshoushan; Anton Hermann

Research Article

Polyamide Dendrimers and Spermine Interact with DNA

Ali Elejmi¹, Asma Abdalgader¹, Wael Elhrari², Inass A Sadawe³, Abdulathim A Alshoushan⁴, Nisreen H Meiqal³, Jamal Ali Elbakay³, Salah M Bensaber³, Anton Hermann⁵ and Abdul M Gbaj³*

¹Department of Chemistry, Faculty of Science, University of Tripoli, Libya

²Polymer Research Center, Libya

³Department of Medicinal Chemistry, University of Tripoli, Libya

Food and Drug Control Centre (LFDA), Libya

⁵Department of Biosciences, University of Salzburg, Austria

Corresponding Author

Received Date: November 28, 2019; Published Date: December 06, 2019

Abstract

Polyamines and polyamide dendrimers based on tetraethyl-1,1,3,3-propanetetracaroxylate positively charged organic cations under physiologic ionic- and pH conditions and therefore interact with negatively charged sites at double stranded Deoxyribo nucleic Acid(dsDNA). The interaction of these dendrimers with the DNA double helix has been studied with two generations (G): G2-NH2, G4-NH2. The binding properties of the two generations were examined by ethidium bromide (EtBr) displacement using fluorescence spectroscopy. It has been shown that G2-NH2 and G4-NH2 dendrimers can interact with dsDNA through an intercalative binding mode as its displaced EtBr from the dsDNA-dye complex. The electrostatic interaction is the main mode of polyamine-nucleic acid interactions. In conclusion, the synthetic dendrimer polymers appear most interesting and highly promising candidates as DNA-interactive drugs that have antitumor activity.

Keywords: DNA-interactive agents; Dendrimers polymer, Chemotherapeutic intervention, Ethidium bromide

Article Details

Citation

Abdul M Gbaj, Ali Elejmi, Asma Abdalgader, Wael Elhrari, Inass A Sadawe, Abdulathim A Alshoushan, et al. Polyamide Dendrimers and Spermine Interact with DNA. Arch Biomed Eng & Biotechnol. 3(3): 2019. ABEB.MS.ID.000563.